COVID-19 Detection Kit Nucleic acid detection kit
Real-time fluorescent RT-PCR kit for detecting 2019-nCoV
【Principle of the procedures】
The kitis based on invitro RT-PCR combining fluorescent probing.Primers and a sequence-specific fluorescence probes wered esigned tailored to high conservative region in 2019-nCoV genome. The probes are oligo nucleotide attached fluorophores at the 5'end with FAM asreporter and 3’endwith quencher.In a mean time,specific primers and probes were developed as internal reference with fluorophores VIC/HEX at tached at 5’end as reporter. During the PCR procedures,the DNA polymerase cleaves the probe at the 5’ end and separates the reporter dye from the quencher dye when the probes hybridize to the target DNA.This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. Monitoring the fluorescence intensities during Real Time allows the qualitative detection of 2019-nCoVin specimens.